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Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques
M.G. Bottone,G. Santin,C. Soldani,P. Veneroni
European Journal of Histochemistry , 2012, DOI: 10.4081/ejh.2012.e4
Abstract: Poly(ADP-ribose) polymerases are a family of enzymes that catalyze the conversion of NAD+ into ADP-ribose. Among them, Tankyrases have been found to bind to centrosome, mitotic spindle and microsome proteins, in the cytoplasm, and to telomeres in the nucleus, where they play a relevant role in telomere metabolism. However, their precise intracellular localization during interphase has not been so far fully elucidated. We investigated this aspect in situ by double immunofluorescence experiments using antibodies recognizing Tankyrases 1-2 or other proteins residing in specific organelles (Golgi apparatus, mitochondria, lysosomes, endoplasmic reticulum). We used HeLa cells as a model system in vitro, before and after treatment with either actinomycin D or etoposide, to also investigate the possible relocation of Tankyrases during apoptosis. We observed that Tankyrases are distributed both in the nucleus and in the cytoplasm; in this latter compartment, they were found to colocate with the Golgi apparatus but never with the mitochondria; a pool of Tankyrases also colocates with the endoplasmic reticulum and lysosomes. Interestingly, in cells with clear signs of apoptosis, Tankyrases were detectable in the cytoplasmic blebs: this suggests that they are not massively cleaved during apoptosis and persist in the largely heterogeneous apoptotic remnants which are known to contain components of cytoplasmic and nuclear origin.
Nuclear localization of phosphorylated c-Myc protein in human tumor cells.
C. Soldani,M.G. Bottone,M. Biggiogera,C. Alpini
European Journal of Histochemistry , 2010, DOI: 10.4081/1750
Abstract: Using immunocytochemical techniques at light and electron microscopy, we analysed the distribution of phosphorylated c-Myc in actively proliferating human HeLa cells. The distribution pattern of c-Myc was also compared with those of other ribonucleoprotein (RNP)-containing components (PANA, hnRNP-core proteins, fibrillarin) or RNP-associated nuclear proteins (SC-35 splicing factor). Our results provide the first evidence that phosphorylated c-Myc accumulates in the nucleus of tumor cells, where it colocalizes with fibrillarin, both in the nucleolus and in extranucleolar structures.
Analysis of ERK3 intracellular localization: dynamic distribution during mitosis and apoptosis | European Journal of Histochemistry
F. Aredia,M. Malatesta,M.G. Bottone,P. Veneroni
- , 2015, DOI: https://doi.org/10.4081/ejh.2015.2571
Abstract: Extracellular signal-regulated kinases (ERK) 1, 2 and 3 are involved in cell proliferation and differentiation, and apoptosis; although ERK1/2 have been widely studied, limited knowledge on ERK3 is available. The present work aimed at investigating ERK3 distribution during cell cycle and apoptosis in human tumor HeLa cells. The analysis performed by double immunofluorescence and immunoelectron microscopy experiments revealed that during interphase ERK3 is mainly resident in the nucleoplasm in association with ribonuclear proteins involved in early pre-mRNA splicing, it undergoes cell cycle-dependent redistribution and, during apoptosis, it remains in the nucleus in the form of massive nuclear aggregates, then moves to the cytoplasm and is finally extruded
Magneto Hydrodynamic Orthogonal Stagnation Point Flow of a Power-Law Fluid Toward a Stretching Surface  [PDF]
Manisha Patel, M.G Timol
American Journal of Computational Mathematics (AJCM) , 2011, DOI: 10.4236/ajcm.2011.12013
Abstract: Steady two dimensional MHD stagnation point flow of a power law fluid over a stretching surface is investigated when the surface is stretched in its own plane with a velocity proportional to the distance from the stagnation point. The fluid impinges on the surface is considered orthogonally. Numerical and analytical solutions are obtained for different cases.
Hemodynamic and hormonal actions of adrenomedullin
Nicholls, M.G.;
Brazilian Journal of Medical and Biological Research , 2004, DOI: 10.1590/S0100-879X2004000800016
Abstract: adrenomedullin, a 52-amino acid residue peptide, has numerous biological actions which are of potential importance to cardiovascular homeostasis, growth and development of cardiovascular tissues and bone, prevention of infection, and regulation of body fluid and electrolyte balance. studies in man using intravenous infusion of the peptide have demonstrated that, at plasma levels detected after myocardial infarction or in heart failure, adrenomedullin reduces arterial pressure, increases heart rate and cardiac output, and activates the sympathetic and renin-angiotensin systems but suppresses aldosterone. the thresholds for these responses differ, being lower under some experimental circumstances for arterial pressure than for the other biological effects. adrenomedullin administration inhibits the pressor and aldosterone-stimulating action of angiotensin ii in man. by contrast, the pressor effect of norepinephrine is little altered by concomitant adrenomedullin administration. although in the absence of a safe, specific antagonist of the actions of endogenous adrenomedullin it is difficult to be certain about the physiological and pathophysiological importance of this peptide in man, current evidence suggests that it serves to protect against cardiovascular overload and injury. hope has been expressed that adrenomedullin or an agonist specific for adrenomedullin receptors might find a place in the treatment of cardiovascular disorders.
Un caso de cefalea post traumática: consideraciones sobre clasificación, fisiopatología y tratamiento
Meli,M.G.;
Revista de la Sociedad Espa？ola del Dolor , 2010,
Abstract: case report of a 35 year old woman with a post traumatic headache (pth). onset of the pain three years earlier, after falling on the ground, which required resuscitation. she had to be on mechanical ventilation for 11 days and suffered several contusions on the right frontal-temporal area of the skull. on discharge, she complained of a continuous bilateral tension headache; she underwent diagnostic cervical medial branch blocks and bilateral mayor occipital nerve blocks with no findings. subsequently, a c7 stellate ganglion block (sgb) on the right side was performed with full pain relief on the same side. headache is the most prevalent symptom of post concussion syndrome. diagnostic criteria for chronic pth secondary to moderate traumatic brain injury are described in the discussion. the pathophysiology of pth is poorly understood. neuroimaging and experimental models offer novel theories to explain a common mechanism for pain in pth and primary headaches. it is assumed that a central and a peripheral sensitization may act through a common pain pathway such as the cervico-trigeminal relay. after a brain injury, pain pathways may be damaged at different levels, causing different types of pain. a clinical case is described here, in which pharmacotherapy, cervical medial branch blocks and occipital nerve blocks failed. on the other hand, sgb was effective, the reason for which might be found in the pathophysiology of the pth, as it causes a temporary block of all the sympathetic fibres to the head and neck. if pth shared the peripheral sensitization mechanism and the vasoregulation impairment with primary headaches, analgesic properties from cervicothoracic sympathetic block would be explained. this unexpected effect should stimulate a review of the international classification of headache disorders ii. stellate ganglion block should be considered as a diagnostic and therapeutic option in case of resistant headache.
Micro Credit Supply by Country Women Association of Nigeria (COWAN) to Rural Women in Ondo State, Nigeria
M.G. Olujide
Research Journal of Applied Sciences , 2012,
Abstract: This study examined the micro-credit given to rural women by Country Women Association of Nigeria (COWAN) in Ondo State. One hundred and six rural women were selected in four local government area of Ondo State, using multi-stage random sampling technique. The study revealed that majority of the respondents age ranges between 21-31 years and 66% were married. The result further showed that respondents` attitude towards COWAN micro-credit are mostly favourable. The constraints encountered by the respondents include inadequate funding, problem of repayment of loan and inability of rural women to pay back the loan. Finally, the chi-square revealed that age, marital status, educational level and religion have no significant relationship with micro-credit received from COWAN organization.
Co-Operative Occupational Education: A Tool for Preparing the Nigerian Youth for Employment in Agriculture
M.G. Olujide
Agricultural Journal , 2013,
Abstract: This study looks at the concept of cooperative occupational education and its needs in Nigeria. It also examined the justifications for cooperative occupation experience programme, need for cooperation between the school and the employers and how best to plan, execute and evaluate cooperative occupation education programmes in Nigeria. It highlighted the need and significance of partnership between industry and education for the purpose of generating an essential skilled work force for Nigeria. It revealed that, vocationally oriented classrooms instruction coupled with a well planned cooperative occupational experience programme will go a long way in preparing the Nigerian youth for entry and advancement in agricultural and related occupations thereby reducing the drop-out, unemployment rates and also making the Nigerian youth marketable in the world of work.
Changing in basic determinants of quality of life in people of working age with chronic skin diseases
Yeremina M.G.
Saratov Journal of Medical Scientific Research , 2012,
Abstract: A comparative assessment of health-related quality of life in working age patients with various chronic diseases of skin was carried out in the given research. Two specifc questionnaires DLQI and DSQL were used. DSQL allows us to distinguish the quality of life in patients with various types of dermatitis using more criteria than the questionnaire DLQI (Dermatology Life Quality Index). There has not a comparative analysis of the usage of these questionnaires in patients with different nosological forms of skin diseases been organized. Aims. The study presents a comparative evaluation of health-related quality of life in patients of working age with psoriasis and atopic dermatitis using two specifc questionnaires — DLQI and DSQL. Methods. The work is based on Saratov State Medical University n.a. VI Razumovsky of Ministry of Health and Social Development of Russia, using the resources of Hematology and Pathology Clinic, Department of Clinical Immunology and Allergy, Department of Skin and Venereal Diseases. To identify the prevalence of disease, employable contingent working at factories with no occupational exposures. An estimating of QoL (quality of life) included interviews with questionnaires written in by patients themselves. The obtained data were statistically processed. Results. Quality of life in patients with psoriasis and atopic dermatitis is signifcantly reduced, even in a period of stable remission. The decline in the quality of life according to the most of scales in patients with atopic dermatitis is more signifcant than with psoriasis. Even non-acute phase of chronic dermatitis (atopic dermatitis and psoriasis) is able to infuence the decrease in productivity at work, has a negative impact on daily activities.
Vocaal-harmonie in de Maleise kern van de Indonesische vocabulaire
M.G. Emeis
Bijdragen tot de Taal-, Land- en Volkenkunde , 1955,
Abstract:
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